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News Releases



14. May 2014


R-Biopharm News Food & Feed Analysis l/2014


  • RIDASCREEN® Sesame Art. No. R7202
  • RIDASCREEN® T-2 / HT-2 Toxin Art. No. R3805
  • New applications for the screening of chloramphenicol in wine
    and enzymes
  • FSA collaborative study – R-Biopharm allergen kits show
    excellent recoveries
  • SureFood® PCR products from our partner
    CONGEN Biotechnologie GmbH, Berlin
  • RIDA®SOFT Win / RIDA®SOFT Win.net Art. No. Z9996
  • Information from R-Biopharm Rhône,
    Scotland
  • Fairs and conference


     Download


 

 6. May 2014


RIDASCREEN®Bacitracin: Quantitative of Bacitracin

 

Screening test for bacitracin in milk, meat, eggs, feed and urine

RIDASCREEN®Bacitracin is a competitive enzyme immunoassay for the quantitative screening of bacitracin in milk, meat, eggs, feed and urine.The test kit contains all reagents required for 96 determinations. As the maximum residue limits of the European Union and the Russian customs union are within the standard range and the detection limits are below these regulatory limits, the test kit allows a sensitive and simple analysis at the same time.

The test has no significant cross reactivity to other antibiotics and is therefore highly specific for bacitracin. The results can be evaluated with the latest RIDA®SOFT Win / RIDA®SOFT Win.net (version: 1.86), which is available on request.

 

Background Information
Bacitracin is a broad spectrum antibiotic and belongs to the group of polypeptides. In addition to veterinary application, bacitracin can be used as antimicrobial growth promoter in animal husbandry. Veterinary drug residues in foodstuff of animal origin and the improper use of antibiotics which is promoting antibiotic resistance of bacteria may pose health risk for the population. Consequently, Bacitracin has been deleted by the Regulation 2821/98/EC from the list of approved animal feed additives (Directive 70/524/EEG Appendix B) and maximum residue limits in foodstuffs have been set in the European Union (bovine milk: 100 ppb) and in the Russian customs union (20 ppb in all food producing species).

RIDASCREEN® Bacitracin (Art. No 2901):
Download Instructions for Use
Download Validation Data

For more information, please contact our international sales office: sales(at)r-biopharm.de




 5. May 2014


Compact Dry: Food Microbiology Testing Made Easier


Compact Dry plates can be used everywhere at any time since the dry plates are not reconstituted before the sample implementation and are therefore stable at common temperatures for long periods of time. Chromogenic agents present in all Compact Dry media types enable easy counting of colonies after incubation.Volume 1: Food Safety Applications Overview

Good understanding of microbiological aspects of food production as well as awareness of possible microbial contaminations of foodstuffs is crucial for the general food safety. Reliable tests systems for analytical evaluation of microbial safety shall ensure delivery of safe products to the consumers and decrease the risk of contamination with life-threatening bacteria.

Compact Dry Food Microbiology Made Easier series will focus on the current and arising issues within the food microbiology and hygiene testing fields subsequently addressing the most common questions and pinpointing the usage details which are critical for the microbiological safety of food. By elaborating on the available analytical solutions, Compact Dry Food Microbiology Made Easier guide will therefore serve as a comprehensive know-how base for all practicing food microbiologists and food safety professionals.

Microbial contaminants in foodstuffs and food production

The problem of microbial contaminants in foodstuffs is not a new one and has been getting more and more into the focus of public health. Even if the consumers do not know much about microorganisms in general they already understood that microbiological testing is necessary to avoid infections caused by foodborne pathogens.

Most common microbiological contaminants in food

The term “salmonella” seems to ring a bell while discussing food safety with laypeople. Salmonella is, yet, only one of numerous parameters which have to be tested in relevant matrices – even if it is one of the most commonly known pathogens. The next group of interest is the “fecal contaminant bacteria” which are exemplified by coliform bacteria, Enterobacteria or E. coli which is still the number one indicator for fecal contaminations.

Other organisms present in the environment of food production facilities, such as Staphylococci, Listeria, Bacilli and last but not least yeasts and molds, could pose additional threats if they are not monitored well. All of them are naturally present on raw materials or humans and some of them have a highly pathogenic potential and can lead to serious diseases.

Finally, the main parameter a food microbiologist has to look for is the total amount of bacteria, the so called “total viable count”.

Microbial contamination monitoring

The most efficient way to monitor the production process on microbial contaminants is the implementation of in-process controls at the production site. These controls mitigate problems at an early stage when set up in the right way: taking samples at critical control points and analyzing them with reliable microbiological methods. Such a control system allows the producer to react quickly and take the right measures to ensure the food safety for the end consumers.

A preliminary option to optimize the process is the control of the incoming raw materials. On the other hand, the end product controls are usually done by accredited laboratories but could be also conducted “in-house” with officially validated and easy to handle methods. This is the mission of the portfolio of Compact Dry: a robust, easy to handle microbiological test system with a proven reliability by official validations according to ISO 16140 as well as AOAC-RI standards.

Compact Dry Plates for Food Microbiology Testing

Compact Dry plates can be used everywhere at any time since the dry plates are not reconstituted before the sample implementation and are therefore stable at common temperatures for long periods of time. Chromogenic agents present in all Compact Dry media types enable easy counting of colonies (product example: Compact Dry Total Count) after incubation.

R-Biopharm offers the analytical test system Compact Dry for the following parameters:

  • Total count
  • Coliform Bacteria
  • E. coli and coliforms
  • Yeasts and Molds
  • Listeria
  • Vibrio
  • Samonella
  • Enterobacteriaceae
  • Enterococcus
  • Staphylococcus aureus
  • Bacillus cereus

Compact Dry provides alternative solutions to classic agar plates and with the following issues of “Compact Dry: Food Microbiology Made Easier” we intent to bring you step by step to this fine product by sharing a deeper insight into the necessity and advantages of microbiological food controls.



25. April 2014

Mycotoxin survelliance in Ecuador and Qatar

A steady stream of publications continues to confirm the widespread occurrence of mycotoxins and builds on existing knowledge. Oritz et al., (December 2013) in Food Additives & Contaminants have reported the co-occurrence of mycotoxins in rice, oat flakes and wheat noodles which are stable foods in Ecuador. Using the R-Biopharm OHCRAPREP® immunoaffinity columns, ochratoxin A was found at levels as high as 93 ng/g in wheat noodles and 161 ng/g in oat flakes.

http://www.tandfonline.com/doi/full/10.1080/19440049.2013.853228#.Uuk9aPunxiw

Hammami et al. (2014) in Food Control have analysed spice samples from a local market in Dohar both for the presence of fungi and for aflatoxins. Using R-Biopharm AFPAPREP® immunoaffinity columns they found levels of 18.5 ng/g aflatoxin B1 in Tandoori masala, 69 ng/g in chilli pepper whilst only 1.8 ng/g aflatoxin B1 in black pepper this sample contained 70.3 ng/g of aflatoxin G2.


http://www.sciencedirect.com/science/article/pii/S0956713513004738



4. April 2014

Scientists discover simpler vitamin analysis

Scientists from Sun Yat-Sen University (Guangzhou, China) have developed an improved procedure for water-soluble multi-vitamin analysis. Heng Zhang et al. in the June 2014 issue of Food Chemistry report a novel extraction buffer which enables efficient simultaneous co-extraction of vitamin B12, folic acid and biotin from infant formula.

 http://dx.doi.org/10.1016/j.foodchem.2013.12.088

These authors employed this buffer in conjunction with R Biopharm VitaFast® test kits obtaining impressive performance in terms of quantifying these vitamins compared with conventional microbiological analysis.  A strong case is made for moving towards multi-vitamin analysis, which is consistent with the strategy being developed by R Biopharm Rhône with the development of a new multi-vitamin immunoaffinity column for vitamin B12, folic acid and biotin.  The R Biopharm team will shortly be publishing the results of their single laboratory validation of this novel immunoaffinity column for multi-vitamin analysis.

EFSA Assesses EU Limits for Deoxynivalenol in Cereals

21. March 2014

EFSA Assesses EU Limits for Deoxynivalenol in Cereals

EFSA has recently delivered an Opinion on the risks for public health related to a possible increase of the maximum level (ML) of deoxynivalenol (DON) for certain semi-processed cereal products from 750 µg/kg to 1000 µg/kg. Based on modelling different scenarios, it was concluded that an increase of the DON maximum levels would increase exposure and consequently increase exceedances of the group health based guidance values for this mycotoxin. The EFSA Panel did however see a need for more occurrence data on DON acetyl-derivatives, using sufficiently sensitive methods in order to assess their impact on the health risk associated with an increase of the maximum levels for DON.


http://www.efsa.europa.eu/en/efsajournal/pub/3490.htm


R-Biopharm DONPREP® immunoaffinity columns offer highly specific clean-up for both DON and 3-acetyl DON for use with HPLC and LC-MS/MS. The complementary DZT MS-PREP® column offers clean-up to those laboratories wanting to monitor not only DON, but also zearalenone and T-2 and HT-2 Fusarium toxins. These columns are widely used in food control enabling official laboratories to meet method performance requirements set out in EU Regulations.


Training Videos on Gluten Free Analysis

A major problem of gluten analysis is the background contamination of cereal dust in the laboratory. Therefore, it is of prime importance to clean the lab with 60 % Ethanol before starting the analysis. The following training videos provide help for carrying out gluten analysis using the R5 dip stick RIDA®QUICK Gliadin in combination with Cocktail (patented) or ethanol extraction. These videos will also help to explain about these two important types of extraction:



These videos will show you, how to analyse samples for their gluten content, first with the sandwich ELISA RIDASCREEN® Gliadin for processed and unprocessed samples and second with the ELISA RIDASCREEN® Gliadin competitive for hydrolyzed or fermented samples like beer, starch, syrup or sour dough:



The last video shows the procedure of allergen FAST ELISAs, as an example the analysis with the RIDASCREEN®FAST Casein is shown:





17. February 2014

Multiplex real-time PCR test kit beef-sheep-goat-small and large ruminants



Particularly innovative:  the first ANIMAL ID Multiplex real-time PCR test kit beef-sheep-goat

 

Multiplex real-time PCR test kit beef-sheep-goat- small and large ruminants in combination with an internal amplification and animal control (IAAC). A test e.g. for feed or even meat analysis.

Using a corresponding 4-channel qPCR thermocycler with the channels FAM, ROX, VIC/HEX and Cy5, three ruminant can be specifically identified in one reaction as well as an internal amplification control is provided.

Additionally, in the absence of such species it can be observed, whether another species is included in the sample. This analysis is not only of importance for correct labeling of meat and meat products: After an intensive but successful eradication of the BSE crisis and an expected increased use of bone meal in the future, it will be increasingly important to test for the absence of ruminant feed material.


Productinformation for the Multiplex real-time PCR test kit beef-sheep-goat




12. December 2013


New RIDASCREEN® β-Agonists ELISA




β-Agonists such as Clenbuterol, Salbutamol and Ractopamine shift energy intake from fat deposition to muscle formation. Therefore, β-Agonists can be used as growth promoters (‘finishers’) in animal farming. However, the use of Clenbuterol and Salbutamol is worldwide prohibited in food producing animals, with some exceptions for veterinary purposes.

R-Biopharm offers a new RIDASCREEN® β-Agonists ELISA for the detection of Clenbuterol (Specificity: 100 %) and Salbutamol (Specificity: 90 %) in various matrices. The new test has improved detection limits for urine and meat. Besides sample preparation methods for serum and feed, the test kit manual includes two new methods for liver and milk.

The new RIDASCREEN® β-Agonists (Art. No. R1704) replaces the previous test (Art. No. R1703). A new version of the RIDA® SOFT Win (1.85) is available on request.


RIDASCREEN® β-Agonists
Download Instructions for Use
Download Validation Data


For spiking, RIDA® β-Agonists Clenbuterol-Spiking Solution is available:
Download Instructions for Use


For the analysis of Ractopamine, RIDASCREEN® Ractopamin is available.


For more information, please contact our international sales office: sales(at)r-biopharm.de


10. December 2013

R-Biopharm News Food & Feed Analysis IV/2013


  • VitaFast® Vitamin C (L-Ascorbinsäure / L-Ascorbic Acid)
  • RIDA®COUNT Total 24
  • RIDA®COUNT – use for cosmetics
  • Compact Dry X-BC validated and certified
  • bioavid News
  • RIDA®SOFT Win / RIDA®SOFT Win.NET Art. No. Z9996
  • News from our cooperation partner GEN-IAL GmbH
  • Information from R-Biopharm Rhône, Scotland
  • Fairs and conferences
  • Workshops 2014


Download



2. December 2013


New applications for the screening of chloramphenicol in wine and enzymes



Since September 2013, residues of the antibiotic chloramphenicol have been detected in various imported enzyme products by the Rapid Alert System for Food & Feed of the European Union (RASFF). As the enzyme products were intended to be used in production of food and drinks (e.g. wine), the occurrence of chloramphenicol traces can, despite the use of the enzymes in small amounts, not completely excluded. Although it is assumed that there is no direct health risk for the consumer of potentially contaminated foodstuff at the moment, the laws of the European Union demand, that food must be completely free of prohibited substances such as chloramphenicol at any time. Therefore, R‑Biopharm developed a new method for the screening of chloramphenicol in enzymes, wine and grape juice with detection limits below the Minimum Required Performance Limit (MRPL) of 300 ng/kg (ppt).

The combination of immunoaffinity clean-up by RIDA® Chloramphenicol column (R1508) and analysis by RIDASCREEN® Chloramphenicol ELISA (R1505) allows an easy and sensitive screening of enzymes, wine and grape juice for residues of chloramphenicol.


Download Application Note and Validation Report for wine and grape juice


Download Application Note and Validation Report for enzymes


R-Biopharm Rhone is currently preparing a sample preparation protocol for the confirmatory analysis of chloramphenicol in wine by LC/MS. For inquiries please contact: info@r-biopharmrhone.com


 

 

 

R-Biopharm Group purchases a majority ownership in Trilogy Lab, expanding US operations.

 

For Immediate Release:

Darmstadt, Germany , April 15, 2010 - R-Biopharm AG, a leading developer of test solutions for clinical diagnostics and food and feed safety has announced today that the company has purchased a majority ownership position in Trilogy Analytical Laboratory, Inc. based in Washington, Missouri. Founded in 1999, Trilogy Analytical is considered the premier commercial testing laboratory for the detection of mycotoxins and other contaminants for food and feed safety. Trilogy’s current facility has expanded into a 22,000 sq. ft. state of the art laboratory facility that employs 28 people. Trilogy’s analytical technology includes LC-MS-MS, HPLC and GC-MS. In addition, Trilogy offers a consumable product line that includes purification columns, chromatography standards and certified reference materials for use in the detection of mycotoxins. “The management team and the senior technical staff will remain in place and Trilogy will continue to operate independently” according to Dr. Ralf Dreher, Managing Director of R-Biopharm AG. “Trilogy’s name and reputation are synonymous with analytical excellence and the retention of Trilogy’s management team was mandatory for our long term strategic plans for expansion into the laboratory service business that will also support our diagnostic test kits. Trilogy is a welcome addition to the R-Biopharm Group”.

R-Biopharm AG currently has 13 subsidiary companies and distributes its products in more than 120 countries. R-Biopharm, Inc. the US subsidiary will relocate and combine its facilities and operations with Trilogy in Washington, MO., although the two companies will continue to operate independently. “It’s important for Trilogy to maintain independence as a reference laboratory” said Kurt Johnson, President of R-Biopharm, Inc. “Even though R-Biopharm Group owns a majority interest in Trilogy they must maintain a level of trust and ensure confidentiality with their clients”.

“R-Biopharm’s commitment to allow us to continue to operate under the Trilogy brand and their engagement of our long term employees was instrumental in our decision to partner with R-Biopharm”, said Carrie Maune, President of Trilogy Analytical. “And although we had discussions with several other fine companies that had an interest in acquiring our business, the long term growth, expansion and independence of Trilogy was paramount in our decision process. R-Biopharm’s vast network of subsidiary companies and distributors will immediately expand our potential customer base worldwide”. R-Biopharm is a leading developer of test solutions for clinical diagnostics and food & feed safety analysis. R-Biopharm assays offer high precision and accuracy, key requirements where patient, consumer and animal health are at risk. R-Biopharm has earned high accolades for its innovation and business performance. R-Biopharm’s industry leading technology has earned the company international recognition by being nominated as a finalist for 'Ernst & Young Entrepreneur of the Year’ in 2004, 2005, 2006 and 2009. R-Biopharm was also recognized in 2004 and 2005 after being placed in the 'Technology Fast 50', a ranking of fastest-growing technology companies.

Please visit our website at www.r-biopharm.com for more information.